Flychip Site Map:
- Home
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- Protocols
- Spotting Libraries
- Sonicated salmon sperm DNA was random prime labelled and used as a landmark
- PCR amplification of the Arabidopsis spike controls
- Linearisation of Drosophila control vectors and PCR of Drosophila control genes
- Transfering 96-well PCR reactions to 384-well microtitre plates
- Adding spotting buffer to the oligonucleotide library
- Robotic Spotting
- Library dessication and rehydration
- Introduction to printing microarrays
- Microarray instrument optimisation
- Printing microarrays with a BioRobotics MicroGrid II 600 or 610 spotter
- Printing microarrays with a Genetix Qarray2 spotter
- Processing Full Moon Biosystems cDNA slides after spotting
- Processing Full Moon Biosystems PowerMatrix slides after spotting
- Processing Amersham CodeLink slides after spotting
- Quality control by staining with SYBR_555
- A key to standard microarray spot identity tracking file formats
- Gene Expression
- In vitro transcription of spike controls from 5'-T7 and dT(15)-3' PCR Products
- Small scale RNA extraction using TRIzol
- Medium scale RNA extraction using TRIzol
- Large scale RNA extraction with TRIzol
- RNA quality control before labelling
- Reverse transcription and direct labelling for cDNA and oligo arrays
- Reverse transcription and indirect labelling for cDNA and oligo arrays
- Klenow labelling of double stranded DNA derived from 3 to 5 µg total RNA
- Anti-sense strand amplification and direct labelling for cDNA microarrays
- Anti-sense strand amplification of RNA and indirect labelling of amino-allyl RNA for oligo microarrays (1 round)
- Anti-sense strand amplification of RNA and indirect labelling of amino-allyl RNA for oligo microarrays (2 rounds)
- Measuring dye incorporation rates
- ChIP-array
- Preparation of fixed chromatin from Drosophila embryos
- Quality control of the fixed chromatin from Drosophila embryos
- Chromatin immunoprecipitation (ChIP) from Drosophila embryos
- Quality control of the chromatin immunopurification (ChIP) from Drosophila embryos
- Ligation-mediated PCR of ChIP genomic DNA
- Measuring dye incorporation rates
- Hybridisation
- Image acquisition
- Archive
- Spotting Libraries
- Facility
- Publications
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- Frequently Asked Questions
