Flychip Site Map:
- Home
- Services
- Protocols
- Gene Expression
- In vitro transcription of spike controls from 5'-T7 and dT(15)-3' PCR Products
- Small scale RNA extraction using TRIzol
- Medium scale RNA extraction using TRIzol
- Large scale RNA extraction with TRIzol
- RNA quality control before labelling
- Reverse transcription and direct labelling for cDNA and oligo arrays
- Reverse transcription and indirect labelling for cDNA and oligo arrays
- Klenow labelling of double stranded DNA derived from 3 to 5 µg total RNA
- SMART amplification
- Anti-sense strand amplification and direct labelling for cDNA microarrays
- Anti-sense strand amplification of RNA and indirect labelling of amino-allyl RNA for oligo microarrays (1 round)
- Anti-sense strand amplification of RNA and indirect labelling of amino-allyl RNA for oligo microarrays (2 rounds)
- Measuring dye incorporation rates
- Hybridisation of labelled material to cDNA microarrays using a Genomic Solutions hybridisation station with Ambion Hybridisation Buffer #1
- Hybridisation to amino-modified long oligonucleotide microarrays using a Genomic Solutions hybridisation station with the Biosolutions hybridisation buffer
- Operating instructions for the Genepix 4000B dual laser scanner
- ChIP and DamID
- ChIP for fly genomic DNA using Affymetrix arrays
- ChIP for fly genomic DNA using Nimblegen arrays
- DamID with Affymetrix microarrays
- DamID with Nimblegen microarrays
- Nimblegen processing of ChIP or DamID samples on 2.1M microarrays
- Affymetrix processing of ChIP or DamID DNA GeneChip Drosophila tiling 2.0R arrays
- Robotic Spotting
- Adding spotting buffer to the oligonucleotide library
- Library dessication and rehydration
- Introduction to printing microarrays
- Microarray instrument optimisation
- Printing microarrays with a BioRobotics MicroGrid II 600 or 610 spotter
- Printing microarrays with a Genetix Qarray2 spotter
- Processing Full Moon Biosystems cDNA slides after spotting
- Processing Full Moon Biosystems PowerMatrix slides after spotting
- Full Moon Biosystems microarray QC kit
- A key to standard microarray spot identity tracking file formats
- Archive
- Slides can be coated with poly-l-lysine before printing cDNA clones
- Transfering 96-well PCR reactions to 384-well microtitre plates
- Sonicated salmon sperm DNA was random prime labelled and used as a landmark
- PCR amplification of the Arabidopsis spike controls
- Linearisation of Drosophila melanogaster control vectors and PCR of Drosophila melanogaster control gene
- Processing poly-l-lysine slides after spotting
- Processing Amersham CodeLink slides after spotting
- Random 9mer oligo hybridisation [array quality control]
- SYTO 61 staining [array quality control]
- Quality control by staining with SYBR_555
- Hybridisation to NH[4] long-oligo arrays using a Genomic Solutions hyb. station
- Hybridisation of labelled material to cDNA microarrays using a Genomic Solutions Hybridisation Station
- Hybridisation to unmodified gDNA microarrays using a Genomic Solutions hybridisation station
- Operating instructions for the arrayWoRx[e] autoloader CCD scanner
- Preparation of fixed chromatin from Drosophila embryos
- Quality control of the fixed chromatin from Drosophila embryos
- Chromatin immunoprecipitation (ChIP) from Drosophila embryos
- Quality control of the chromatin immunopurification (ChIP) from Drosophila embryos
- Ligation-mediated PCR of ChIP genomic DNA
- Measuring dye incorporation rates
- Gene Expression
- Facility
- Publications
- Links
- Frequently Asked Questions
