Archive of our out-of-date protocols
Coating standard glass microscope slides with poly-l-lysine:
- Slides can be coated with poly-l-lysine before printing unmodified PCR amplified cDNA clones (html) (PDF)
Making the cDNA libraries:
- Transfering 96-well PCR reactions to 384-well microtitre plates(html) (PDF)
- Sonicated salmon sperm DNA was random prime labelled and used as a landmark (html) (PDF)
- PCR amplification of the Arabidopsis spike controls (html) (PDF)
- Linearisation of Drosophila melanogaster control vectors and PCR of Drosophila melanogaster control gene (html) (PDF)
Microarray processing:
- Processing poly-l-lysine slides after spotting (html) (PDF)
- Processing Amersham CodeLink slides after spotting (html) (PDF)
Microarray quality control:
- Random 9mer oligo hybridisation (html) (PDF)
- SYTO 61 staining (html) (PDF)
- Quality control by staining with SYBR_555 (html) (PDF)
Hybridisation of processed biological materials to a microarray:
- Hybridisation to amino-modified long oligonucleotide microarrays using a Genomic Solutions hybridisation station with our hybridisation buffer (html) (PDF)
- Hybridisation to unmodified gDNA microarrays using a Genomic Solutions hybridisation station (html) (PDF)
- Hybridisation of labelled material to cDNA microarrays using a Genomic Solutions Hybridisation Station (html) (PDF)
Image acquisition:
ChIP:
- Preparation of fixed chromatin from Drosophila embryos (html) (PDF)
- Quality control of the fixed chromatin from Drosophila embryos (html) (PDF)
- Chromatin immunoprecipitation (ChIP) from Drosophila embryos (html) (PDF)
- Quality control of the chromatin immunopurification (ChIP) from Drosophila embryos (html) (PDF)
- Ligation-mediated PCR of ChIP immunopurified genomic DNA (html) (PDF)
- Measuring (nucleic acid concentration and) dye incorporation rates (html) (PDF)
