• CamSysBiol
• Russell Lab
• DrosDel
• FlyMine
• FlyBase
• Proteomics

Chromatin immunopurification (ChIP) and DNA adenine methyltransferase identification (DamID) microarray:

ChIP

• Preparation of fixed chromatin from Drosophila embryos (html) (PDF)
• Quality control of the fixed chromatin from Drosophila embryos (html) (PDF)
• Chromatin immunoprecipitation (ChIP) from Drosophila embryos (html) (PDF)
• Quality control of the chromatin immunopurification (ChIP) from Drosophila embryos (html) (PDF)
• Ligation-mediated PCR of ChIP immunopurified genomic DNA (html) (PDF)
• Measuring (nucleic acid concentration and) dye incorporation rates (html) (PDF)

DamID

• DamID with Affymetrix microarrays (html) (PDF)
• DamID with Nimblegen microarrays (html) (PDF)

Nimblegen microarray hybridisation

• Nimblegen processing of ChIP or DamID samples on 2.1M microarrays (html) (PDF)

Affymetrix microarray hybridisation

• Coming soon!

Get all these protocols under one PDF file:

chip-protocols.pdf  61KB