• CamSysBiol
• Proteomics
• FlyMine
• FlyBase
• INDAC
• DrosDel
• Affymetrix

Gene expression sample processing:

Making the spike control mix:

• In vitro transcription of spike controls from 5′-T7 and dT₁₅-3′ PCR Products (html) (PDF)

RNA extraction and quality control:

• Small scale RNA extraction using TRIzol (html) (PDF)
• Medium scale RNA extraction using TRIzol (html) (PDF)
• Large scale RNA extraction with TRIzol (html) (PDF)
• RNA quality control before labelling (html) (PDF)

Reverse transcription, labelling and amplification:

• Reverse transcription and direct labelling for cDNA and oligo arrays (html) (PDF)
• Reverse transcription and indirect labelling for cDNA and oligo arrays (html) (PDF)
• Klenow labelling of double stranded DNA derived from 3 to 5 µg total RNA (html) (PDF)
• Klenow labelling of double stranded DNA (html) (PDF)
• Anti-sense strand amplification and direct labelling for cDNA microarrays (html) (PDF)
• Anti-sense strand amplification of RNA and indirect labelling of amino-allyl RNA for oligo microarrays (1 round) (html) (PDF)
• Anti-sense strand amplification of RNA and indirect labelling of amino-allyl RNA for oligo microarrays (2 rounds) (html) (PDF)
• Measuring (nucleic acid concentration and) dye incorporation rates (html) (PDF)

Get all these protocols under one PDF file:

gene_expression-protocols.pdf  126KB