Gene expression sample processing:
Making the spike control mix:
RNA extraction and quality control:
- Small scale RNA extraction using TRIzol (html) (PDF)
- Medium scale RNA extraction using TRIzol (html) (PDF)
- Large scale RNA extraction with TRIzol (html) (PDF)
- RNA quality control before labelling (html) (PDF)
Reverse transcription, labelling and amplification:
- Reverse transcription and direct labelling for cDNA and oligo arrays (html) (PDF)
- Reverse transcription and indirect labelling for cDNA and oligo arrays (html) (PDF)
- Klenow labelling of double stranded DNA derived from 3 to 5 µg total RNA (html) (PDF)
- SMART: Atlas Switch Mechanism At the 5' end of Reverse Transcript amplification of RNA(html) (PDF)
- Anti-sense strand amplification and direct labelling for cDNA microarrays (html) (PDF)
- Anti-sense strand amplification of RNA and indirect labelling of amino-allyl RNA for oligo microarrays (1 round) (html) (PDF)
- Anti-sense strand amplification of RNA and indirect labelling of amino-allyl RNA for oligo microarrays (2 rounds) (html) (PDF)
- Measuring (nucleic acid concentration and) dye incorporation rates (html) (PDF)
Hybridisation:
- Hybridisation of labelled material to cDNA microarrays using a Genomic Solutions hybridisation station with Ambion Hybridisation Buffer #1(html) (PDF)
- Hybridisation to amino-modified long oligonucleotide microarrays using a Genomic Solutions hybridisation station with the Biosolutions hybridisation buffer(html) (PDF)
Image aquisition:
Get all these protocols under one PDF file:
gene_expression-protocols.pdf 137KB
