• CamSysBiol
• Russell Lab
• DrosDel
• FlyMine
• FlyBase
• Proteomics

Robotic spotting of microarrays:

Library management

• Adding spotting buffer to the oligonucleotide library (html) (PDF)
• Library dessication and rehydration (html) (PDF)

Instrument optimisation

• Introduction to printing microarrays (html) (PDF)
• Microarray instrument optimisation (html) (PDF)

Microarray printing

• Printing microarrays with a BioRobotics MicroGrid II 600 or 610 spotter (html) (PDF)
• Printing microarrays with a Genetix Qarray2 spotter (html) (PDF)

Microarray processing

• Processing Full Moon Biosystems cDNA slides after spotting (html) (PDF)
• Processing Full Moon Biosystems PowerMatrix slides after spotting (html) (PDF)
• Processing Amersham CodeLink slides after spotting (html) (PDF)

Microarray quality control

• Quality control by staining with SYBR_555 (html) (PDF)

Microarray spot tracking file formats

• A key to standard microarray spot identity tracking file formats (html) (PDF)

Get all these protocols under one PDF file:

robotic_spotting-protocols.pdf  227KB