Full Moon Biosystems QC kit
Overview
After printing, a random sample of microarrays is stained and then scanned so that we can asses the print quality and constancy. These checks include substrate defects, sub-grid and meta-grid positioning on the substrate, checking that all spots have been printed and the spot morphology. The outlined protocol is based on the method recommended by Full Moon Biosystems (http://www.fullmoonbio.com/).
Equipment and Reagents
- Full Moon Biosystems QC kit
- MilliQ water
- Slide staining rack (Philip Harris; Cat. No. B52651)
- Slide staining trough (Philip Harris; Cat. No. B52649)
- Hettich Rotina 35 microtitre plate centrifuge
- Microscope slide box (Merck EuroLab; Cat. No. 406/0286/00)
- Horizontal laminar flow work station (Jencons; Cat. No. 566-031)
- Lint-free tissue
- Standard photographic air duster
Procedure (4 slides, per batch)
- Add 25 mL QC solution to a reaction tube
- Place printed and blocked slides in the reaction tube
- Incubate on an orbital shaker for 1 minute at room temperature
- Dip slides in 250 mL 0.05% SDS/0.1 x SSC (5 times)
- Dip slides in 250 mL 0.1 x SSC (5 times)
- Dip slides in 250 mL MilliQ water (5 times)
- Transfer to a microscope slide box with lint-free tissue in the base
- Centrifuge at 2000 rpm for 2 minutes
- Remove any water droplets from the slide using an air duster
- Scan using the cy3-channel of a CCD or dual laser scanner
R. Auburn (18-08-2008).
