Spotting libraries
Making the cDNA libraries:
- Our cDNA libraries were PCR amplified before spotting in 96-well microtitre plates
- Sonicated salmon sperm DNA was random prime labelled and used as a landmark (html) (PDF)
- PCR amplification of the Arabidopsis spike controls (html) (PDF)
- Linearisation of Drosophila melanogaster control vectors and PCR of Drosophila melanogaster control genes (html) (PDF)
Making the oligonucleotide libraries:
- Oligonucleotide synthesis was performed by a commercial supplier
- Adding spotting buffer to the oligonucleotide library (html) (PDF)
Get all these protocols under one PDF file:
spotting_libraries-protocols.pdf 41KB
