Normalisation:
Correcting systematic biases, e.g., differential dye labelling
The method we employ is closely based on the work published by Huber et al. (2002). For further information on this method please refer to Huber W., von Heydebreck A., Sultman H., Poustka A., and Vingron M. (2002). Variance stabilization applied to microarray data calibration and to the quantification of differential expression. Bioinformatics 18(Suppl.1), S96-S104. PMID 12169536 (abstract).
Normalisation and averaging of an entire replica group
Key
M = channel 2 - channel 1
A = (channel 2 + channel 1) / 2
