Scanning:
Producing the microarray images
Scanning is performed using the Genepix 4000B dual laser scanner (Axon) or the arrayWorX[e] CCD scanner (Applied Precision). In each instance, one 16-bit TIF-format greyscale image is generated per channel for each hybridised microarray. Fluorescent spot signal is then quantified using these grey-scale images with publicly available software, as described below. False colour images are only to be used for presentations or for simple quality control checks, e.g. spot structure, background, etc.
Grey scale and colour images
