ChIP-array:
Identify and map target sites for genomic DNA binding proteins
In conjunction with Rob White’s laboratory, we have developed techniques for combining chromatin immunopurification (ChIP) with DNA microarrays. Our DNA adenine methyltransferase identification (DamID) method is based on the Vogel et. al. (2007) Nat Protoc 2(6):1467-78 protocol (abstract). The FlyChip facility will assist researchers in implementing these technologies, however, in recognition of the unique nature of each ChIP and DamID experiment, we cannot offer these as full services.
At present the assistance we can offer is:
- Access to whole genome Drosophila tiling arrays from Nimblegen
- Technical advice on ChIP and DamID from Drosophila tissues
- Labelling, hybridisation, scanning and basic analysis for researchers who have isolated suitable chromatin (Disclaimer)
- Advice on generating boutique robotic spotting genome tiling arrays
Researchers are strongly advised to contact contact FlyChip before undertaking any experimental work.
