ChIP-array:
Identify and map target sites for genomic DNA binding proteins
In conjunction with Rob White’s laboratory, we have developed techniques for combining chromatin immunopurification from Drosophila tissues with DNA microarrays. The FlyChip facility will assist researchers in implementing this technology, however, recognising the unique nature of every ChIP-array experiment, we cannot offer this as a full service.
At present the assistance we can offer is:
- Provision of pilot Drosophila 4Mb genome tiling microarrays (FG001)
- Access to whole genome Drosophila tiling arrays when they become available
- Provision of cDNA arrays (FC004) for ChIP-array studies, useful for transcriptional regulators binding close to the start of transcription
- Technical advice on chromatin immunopurification from Drosophila tissues
- Labelling, hybridisation, scanning and basic analysis for researchers who have isolated suitable chromatin
- Advice and design on the generation of tiling arrays
Researchers are strongly advised to contact contact FlyChip before undertaking any experimental work.
