Spotted microarrays
| Core Drosophila or custom-printed spotted arrays | Internal (exc. VAT) | External (inc. VAT) |
|---|---|---|
| Drosophila transcriptome long-oligonucleotide microarrays: | £100, per array | £120, per array |
| Custom printing of your spotted microarrays (all species): | Enquire | Enquire |
Gene expression
| Analysis of differential gene expression by microarray | Internal (exc. VAT) | External (inc. VAT) |
|---|---|---|
| Full service - without RNA amplification: | £250, per array | £295, per array |
| Full service - with one round of RNA amplification: | £350, per array | £415, per array |
| Full service - with two rounds of RNA amplification: | £500, per array | £590, per array |
| Custom gene expression experiments: | Enquire | Enquire |
Our full service includes RNA extraction, labelling, hybridisation, scanning, spot-finding, and basic normalisation.
ChIP and DamID
| Identify and map target sites for genomic DNA binding proteins | Internal (exc. VAT) | External (inc. VAT) |
|---|---|---|
| Custom ChIP service: | Enquire | Enquire |
| Custom DamID service: | Enquire | Enquire |
Affymetrix
| Gene expression studies and mapping target sites for genomic DNA binding proteins | Internal (exc. VAT) | External (inc. VAT) |
|---|---|---|
| We hybridise the samples to your microarrays and then add fluorescent labels and scan these microarrays: | £75, per array | £90, per array |
| Users must supply biotin-labelled samples and Affymetrix microarrays: http://www.affymetrix.com/ | ||
| The Affymetrix service is not limited to Drosophila |
Nimblegen
| Mapping target sites for genomic DNA binding proteins | Internal (exc. VAT) | External (inc. VAT) |
|---|---|---|
| Users to supply labeled samples, arrays and mixers. We hybridise your samples to the microarrays, then scan and strip the microarrays: | £75, per array | £100, per array |
| Users to supply arrays and mixers. We label your samples (for NimbleGen HD2 Arrays), then hybridise, scan and strip your microarrays: | £275, per array | £330, per array |
| The Nimblegen service is not limited to Drosophila |
Analysis of spotted microarrays
| Scanning, signal quantification, normalisation, and statistical analyses | Internal (exc. VAT) | External (inc. VAT) |
|---|---|---|
| Array scanning and spot-finding: | £30, per array | £40, per array |
| Additional statistical analysis: | £50, per hour | £65, per hour |
Analysis of Affymetrix microarrays
| Gene expression analyses | Internal (exc. VAT) | External (inc. VAT) |
|---|---|---|
| We can perform gene expression analysis using R: | Enquire | Enquire |
| Analysis tools for Affymetrix arrays are available from affymetrix: http://www.affymetrix.com/ |
Disclaimer
Users submit samples to FlyChip entirely at their own risk, genomics analysis involves complex molecular biology and failures do occur, thus we cannot guarantee that labelling and subsequent hybridisation will yield interpretable data. While we perform a number of QC checks, the cause of some failures may sometimes not be determined. Unless we can unambiguously determine that we have made an error we must charge for all work undertaken.
FlyChip can not be held responsible for experiments that are incorrectly performed or delayed when you did not label your tubes correctly, or tell us what your sample aliquots are, and what each microfuge tube contains. This system has been designed to prevent mistakes and to ensure that the time you spent collecting your samples was not wasted.
Please contact FlyChip if you have any questions about this.
